| abstract |
The present invention provides a novel substantially pure glycosidase suitable as a reagent that provides a purified enzyme preparation in a form having a distinct substrate specificity and reproducible cleavage activity. Glycosidases isolated from Xanthomonas and recombinant glycosidases have been disclosed as substantially pure glycosidases that can cleave selected glycosidic bonds. The substrate specificity of the isolated enzyme is GlcNacβ1-X, Galα1-3R, Galα1-6R, Galβ1-3R, Fucα1-2R, Fucα1-3R, Fucα1-4R, Manα1-2R, Manα1-3R, Manα1-6R , Manβ1-4R, Xylβ1-2R and Glcβ1-4R, resulting in improved ability to selectively cleave glycosidic bonds in carbohydrate substrates and to form modified carbohydrates. [Selection figure] None |