http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008154514-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_24aca9ded2638ea793d05360dde7a4a0 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2006-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b703e1aa6b7af4716e4c64ae7fd317ca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d11c8b1d60b034593e790326ef5742b |
publicationDate | 2008-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2008154514-A |
titleOfInvention | DNA methylation measurement method |
abstract | A method for measuring the content of methylated DNA is provided. SOLUTION: Single-stranded DNA: A is bound to a single-stranded immobilized oligonucleotide B from a sample, A is selected to form double-stranded C, and C is digested with a methylation sensitive restriction enzyme and then generated. The free digested product is removed, the undigested product C is separated into single strands, the generated free single strand DNA and B are combined to select single strand DNA, and the selected free single strand Double strand D formed by extending B once with B DNA as a template using B as a primer: PR is once separated into single strands, and the resulting single strand DNA (positive strand) is bound to B Single-stranded DNA is selected, single-stranded DNA is used as a template, B is PR and B is extended once to form double-stranded DNA, and the resulting single-stranded DNA (negative strand): specific PR using E as a template : F is PR and F is extended once to form E as a double strand Once After separation of double-stranded DNA into single-stranded state they were repeatedly amplified and quantified. [Selection figure] None |
priorityDate | 2006-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 100.