| abstract |
Disclosed is a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) that is not derived from LCR. An isolated polynucleotide, wherein a DNase I hypersensitive site is reproducible for a gene to which the polynucleotide is operably linked with the chromatin open or chromatin open. A polynucleotide comprising a broad unmethylated CpG island and lacking the DNase I hypersensitive site, except when associated with a promoter characterized in that it facilitates expression . [Selection figure] None |