Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ed4faa2bdfe4ddde15d2ec16b7ba4f8b |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 |
filingDate |
2006-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e03ab7406a4ce765652f34a261bcb455 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2baf01562e0e523fa5a9da1be61c9b25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_336ba86888dd7681bffbfc5d7d85ec13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b680dd497883c7804a6592ae2a7a71b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_87bf05bb694e0f04bbce8b0e3c8d8147 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab4cca787949a21c05b9e04200d781d1 |
publicationDate |
2008-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
JP-2008017733-A |
titleOfInvention |
Heterologous protein expression method and protein obtained by heterologous protein expression method |
abstract |
To establish a protein production method capable of expressing two different proteins in one cell and stably performing protein production control under induction conditions. A Pichia methanolica the (Pichia methanolica) as host, introducing the nucleotide sequence encoding a first protein downstream of a P MOD1 promoter, a nucleotide sequence encoding a second protein downstream of P MOD2 promoter Obtaining a recombinant of Pichia methanolica, culturing the recombinant in a culture medium in the presence of oxygen, and in the process, the PMOD1 promoter in the presence of glycerol and / or xylose The method of expressing a heterologous protein comprising the steps of: expressing the first protein in a step; and expressing the second protein in the PMOD2 promoter in the presence of methanol. [Selection figure] None |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020096610-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7308160-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11319544-B2 |
priorityDate |
2006-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |