http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007535928-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2005-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2007-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2007535928-A |
| titleOfInvention | Detection of chromosomal abnormalities |
| abstract | A method for detecting any one of a plurality of chromosomal abnormalities resulting from aneuploidy or specific mutations, particularly microdeletions, in a single assay, and a kit for use therein. In order to amplify eukaryotic genomic DNA, a primer oligonucleotide pair comprising a plurality of primer oligonucleotide pairs, each of which has a detectable label attached to its 5 primer. Perform the reaction (PCR). Multiple primer pairs target DNA segments of various target chromosomes, which are indicators of potential chromosomal abnormalities, and one pair targets a control gene. Purifying the amplified PCR product to obtain single stranded DNA having a detectable label therefrom, each comprising a DNA oligonucleotide probe having a nucleotide sequence complementary to the nucleotide sequence of one strand of each segment; Hybridize with spots on the microarray. The microarray is imaged for the presence of a label on its respective spot, and the absence or presence of a chromosomal abnormality indicated by one or more of the target DNA segments of interest indicates that the imaging results are first specific to the control gene Diagnosis is made by comparing to a spot image and then to a result obtained from normal DNA imaging. |
| priorityDate | 2004-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 206.