| abstract |
An I-DmoI derivative with enhanced cleavage activity at 37 ° C., the variant comprising a variant of I-DmoI endonuclease or a sequence of its chimeric 5 derivative comprising at least a first I-DmoI domain, At least: (i) one of the residues at positions 4, 20, 49, 52, 92, 94 and / or 95 of the above first I-DmoI domain, and / or (ii) a linker or second of I-DmoI Includes substitution of one of the residues at the first position 101, 102 and / or 109 (if present) of the two domains. 10 Polynucleotides encoding the above derivatives, cells, animals or plants containing the polynucleotides and their use to isolate meganucleases having novel DNA target specificity. [Selection] Figure 6 |