| abstract |
The present invention provides dyes, biosensors, and methods of using dyes and biosensors to detect selected target molecules. The biosensor has a binding domain, as well as a dye or a dye that binds directly to the desired target. A binding domain contemplated by the present invention is a biomolecule or biomolecule that interacts with the desired target molecule and can be specific for a given structural state or covalent modification (eg, phosphorylation) of the molecule. Includes molecular fragments. In some embodiments, the binding domain of the biosensor is a combination of a single chain variable region fragment (scFv) and a dye according to the present invention bound to a CDR3 region. The present invention also provides environmentally sensitive dyes useful for detecting changes in binding, structural changes, or post-translational modifications of selected targets. [Selection] Figure 11A |