| abstract |
The present invention relates to mutant adeno-associated, exhibiting altered capsid properties, such as, for example, reduced binding to neutralizing antibodies in serum and / or altered heparin binding and / or altered infectivity of certain cell types. Provide virus (AAV). The present invention further provides a library of mutant AAV comprising one or more mutations in the capsid gene. The invention further provides methods for making mutant AAV and mutant AAV libraries, and compositions comprising mutant AAV. The present invention further provides a recombinant AAV (rAAV) virion comprising a mutant capsid protein. The invention further provides a nucleic acid comprising a nucleotide sequence encoding a mutant capsid protein, and a host cell comprising the nucleic acid. The present invention further provides a method of delivering a gene product to an individual, generally comprising administering an effective amount of a subject rAAV virion to the individual in need thereof. |