| abstract |
The present invention relates to a cell culture medium and a method for culturing cells by continuous perfusion culture of a cell culture comprising cells, wherein the cell culture medium is added to the cell culture, the cell culture comprising hollow fibers. Resulting in the outflow of liquid having a lower cell density than the cell culture, and the flow in the filter module is an alternating tangential flow. Preferably, the culture medium is added at a specific perfusion rate and / or the biomass is removed from the culture at least once. This technique is particularly suitable for culturing aggregated cells. The invention also relates to such a method in which the biological material, preferably antibodies, are produced by the cells and the biological material can be further purified in downstream processing. |