| abstract |
The present invention provides compositions and methods for making translation components that expand the number of amino acids genetically encoded in eukaryotic cells. These components include orthogonal tRNAs, orthogonal aminoacyl tRNA synthetases, orthogonal pairs of tRNA / synthetase and unnatural amino acids. A method for producing a protein and a protein incorporating an unnatural amino acid in a eukaryotic cell is also provided. |