| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6467 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-575
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2004-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2007-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2007524364-A |
| titleOfInvention |
Cleavage of fusion proteins using granzyme B protease |
| abstract |
A method of preparing a polypeptide of interest in authentic form by enzymatic cleavage of a fusion protein using granzyme B protease (EC 3.4.21.79). Also provided is a fusion protein comprising a polypeptide of interest and a fusion partner, wherein the binding region between the polypeptide of interest and the fusion partner includes a granzyme B protease cleavage site adjacent to the polypeptide of interest, and a cysteine residue. Group 228 (chymotrypsinogen numbering) contains a human granzyme B protease variant mutated to phenylalanine. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015532102-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017140051-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2022033246-A |
| priorityDate |
2003-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |