| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-971
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-25375
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-405
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-111666
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-107497
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-113332 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-147
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-536
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5005 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-34 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-536
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate |
2004-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2007-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2007503593-A |
| titleOfInvention |
Method and apparatus for preparing cell samples for intracellular antigen detection using flow cytometry |
| abstract |
Methods and apparatus for preparing biological cell samples for intracellular analysis. The present invention is based on the recognition that many steps of such routine methods for sample preparation are eliminated, and the method itself is automated and comes with its advantages. The methods of the invention include (a) cell immobilization, (b) permeabilization, and (c) staining (or labeling) of the intracellular molecule of interest with a probe that is readily detectable by flow cytometry techniques. All, without the cell washing step (and resuspension) step. Rather, a single cell wash step is affected after these three steps are performed. Preferably, the washing step is performed by passing the immobilized, permeabilized and stained cell sample through a semi-permeable membrane that maintains the cells of interest while filtering and discarding interfering substances. . |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102144165-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015500999-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022085341-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016028251-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016522899-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011527192-A |
| priorityDate |
2003-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |