| abstract |
The present invention relates to a method for producing a polyunsaturated fatty acid in an organism into which a nucleic acid encoding a polypeptide having Δ5-elongase activity has been introduced. The nucleic acid sequence may be advantageously expressed in the organism, optionally together with other nucleic acid sequences encoding polypeptides for fatty acid biosynthesis or lipid metabolism. Particularly advantageous are nucleic acid sequences encoding Δ6-desaturase, Δ5-desaturase, Δ4-desaturase and / or Δ6-elongase activity, preferably the desaturase and elongase are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a process for producing oils and / or triacylglycerides with an increased content of long-chain polyunsaturated fatty acids. Particular embodiments of the present invention are methods for producing unsaturated ω3-fatty acids and triglycerides having an increased content of unsaturated fatty acids (particularly ω3-fatty acids having more than 3 double bonds). Also preferably an ω3-desaturase, for example a fungus of the family Pythiaceae, for example a phytophythora genus, for example the Phytophthora infestans genus / species, or an algae, for example the Prasinophyceae family, for example the Ostreococcus genus, especially the Ostreococcus tauri genus / species, or As a result of the expression of elongase and desaturase used in the method in combination with ω3-desaturase from algae, for example Thalassiosira spp., In particular Thalassiosira pseudonana sp. Also disclosed is the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, having an increased content. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and / or nucleic acid constructs, and transgenic organisms comprising the nucleic acid sequences, nucleic acid constructs and / or vectors. Another aspect of the present invention relates to oils, lipids and / or fatty acids produced by the above methods, and their use, as well as unsaturated fatty acids and triglycerides with increased content of unsaturated fatty acids, and uses thereof About. |