| abstract |
An object of the present invention is to provide a method for suitably quantitatively analyzing gene expression of a single cell. A method of collecting a single cell from a sample containing at least a single cell, a cell lysis step of lysing a cell membrane of the collected single cell and eluting nucleic acid in the cell, and Among them, a DNA degradation step of degrading DNA with a DNA degrading enzyme, a step of hybridizing mRNA of RNA contained in the single cell to oligo (dT) immobilized on a carrier, and hybridizing to oligo (dT) A reverse transcription reaction is performed on the mRNA, and a single cell-derived cDNA-immobilized carrier library is prepared by immobilizing a single-cell-derived cDNA on a carrier, and an amplification reaction is performed on the cDNA immobilized on the carrier. And a step of detecting the amount of amplification. [Selection] Figure 4 |