| abstract |
The present invention relates to a method for measuring hemoglobin using cation exchange liquid chromatography, and particularly provides a method for measuring hemoglobin capable of measuring stable hemoglobin A1c with high accuracy. A method for measuring hemoglobin using cation exchange liquid chromatography, the first wavelength light for detecting hemoglobin, and a wavelength larger than the first wavelength light, and After performing absorbance measurement using a second wavelength light having a wavelength of 450 to 480 nm, a hemoglobin is prepared by calculating a peak ratio by creating a chromatogram from the difference between the absorbance in the first wavelength light and the absorbance in the second wavelength light. Measuring method. [Selection figure] None |