http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007306871-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_52a65b677bbedbe7bf090dd556a7cc43 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
filingDate | 2006-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7357810f10e1d23da08da2a693cd7589 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff43042a5ce79766f05ef5dd49a81dee |
publicationDate | 2007-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2007306871-A |
titleOfInvention | Inclusion body solubilization method and purification method |
abstract | An object of the present invention is to provide a method for solubilizing an Evil luciferase 19 kDa protein expressed in E. coli while maintaining its luminescence activity. An inclusion body obtained by expressing a 19 kDa protein in E. coli is suspended in a buffer containing 2M urea without using guanidine, and after centrifugation, the insoluble precipitate is converted into a buffer containing 6M urea. The 19 kDa protein is solubilized by suspending. [Selection figure] None |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9840730-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017176182-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016144454-A |
priorityDate | 2006-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.