| abstract |
An object of the present invention is to provide a technique for efficiently inducing differentiation of functional cells for tissue regeneration or repair using stem cells isolated from mammalian biological tissue. A technique for preparing functional cells for transplantation with a very high engraftment rate used in regenerative medicine by combining adhesion culture and suspension culture using stem cells isolated from mammalian living tissue. It was. In particular, in the present invention, in the suspension culture step, the cell aggregate (sphere body) has a diameter of 50 to 500 μm, the undifferentiated state of the stem cells is continuously maintained, and the skin functionality is further improved. It showed extremely excellent effects on the differentiation induction efficiency into cells and the survival rate after transplantation. That is, the present invention provides a method for continuously maintaining the undifferentiated state of stem cells and then efficiently inducing differentiation of functional cells for regeneration or repair of tissues with a very high engraftment rate. . |