| abstract |
Provided is a vaccine production method for increasing the expression of a protein antigen in a baculo vector virus expression system. A method for producing a vaccine comprising a classic swine fever virus E2 baculovirus protein comprising the following steps: Selecting recombinant baculoyl encoding the protein, growing insect cells in growth medium in a culture vessel with a sufficient capacity of at least 2 liters, 1 × 10 5 to 1 × 10 6 cells / ml Infecting the cells with a density of less than 0.01 MOI with the inoculum of the baculovirus, culturing the cells until more than about half of the cells show cytopathic effect, and the E2 protein in the growth medium A step of removing cellular substances when the final concentration is 100 to 300 μg / ml, a step of preparing a vaccine without performing immunoaffinity purification. The production method is further applicable to the production of recombinant follicle stimulating hormone. [Selection figure] None |