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publicationDate 2007-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2007244341-A
titleOfInvention Biological cell culture control method and culture control apparatus
abstract [Problem] To maintain a substance required by living cells at an appropriate concentration for culturing, while continuing to cultivate while suppressing accumulation of substances that inhibit growth as much as possible, and to produce target products efficiently A culture control method and a culture control apparatus are provided. In a culture apparatus for culturing a living cell while supplying a substance required by the living cell during culturing, a part of the culture solution is collected and the concentration of the living cell, glucose concentration, glutamine concentration, lactic acid concentration, ammonia Estimate the culture state of the living cells from the measurement values obtained by the measurement means for measuring the concentration and the culture product concentration, calculate at least the supply amount to be supplemented with glutamine, and change the glutamine concentration in the culture solution from 0.16 mM. Control to maintain in the 1 mM range. [Selection] Figure 1
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