| abstract |
[PROBLEMS] To develop a method for easily and accurately assaying the ability of a substance to inhibit drug metabolizing enzymes. [Solution Means] 1) Mammal-derived drug metabolizing enzyme (this enzyme), indicator substrate (this substrate) and this substrate A step of selecting a candidate reaction solution in which the concentration of the substrate in a reaction solution containing a solvent suitable for dissolving the substrate is suitable for generating a metabolite of the substrate, 2) from the candidate reaction solution in the previous step A step of selecting a reaction solution containing a solvent suitable for dissolving the substrate and the substance, and 3) in the reaction solution of the previous step, the substrate, the substance and the enzyme at a concentration corresponding to these 4) a step of producing a metabolite of the substrate by the enzyme while contacting with 4) a step of measuring the amount of metabolite produced in the previous step using an HPLC tandem mass spectrometer, and 5) measurement of the previous step. Based on the production amount or index value, Assay method, such as a drug-metabolizing enzyme inhibitory ability to have a step, for evaluating the like. [Selection figure] None |