| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be055db3c1a09879df07379ba969e223 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-14 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-14 |
| filingDate |
2004-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0fa0e55b1c4161d1ebcad3eea81a8eb7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0fe5e43c7cea4c2022f5d9721cf48c24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56d3d3d053d301c5f764a6760cd2e602
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab3ae9223e91f38da01974a68f4be334
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f84682c1ecb7c5c4720012c556284672 |
| publicationDate |
2007-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2007222003-A |
| titleOfInvention |
Construction of actinomycete H + -pyrophosphatase synthesis gene and expression system in Escherichia coli |
| abstract |
When a wild-type actinomycete H + -PPase gene is introduced into Escherichia coli in the form of a plasmid, the transformed strain grows very badly and the plasmid cannot be stably present. In addition, homologous recombination often occurred, resulting in loss of H + -PPase function. A rare codon for Escherichia coli is replaced with another codon on the condition that the amino acid sequence of the wild-type actinomycete H + -PPase gene sequence does not change. The improved actinomycetes H + -PPase gene thus modified can be stably present on a plasmid in E. coli, and the growth of the transformant is not different from usual. The activity of actinomycetes H + -PPase expressed in this Escherichia coli is maintained. [Selection] Figure 5 |
| priorityDate |
2004-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |