| abstract |
The present invention provides a fed-batch fermentation method using a special host / vector system of E. coli for the production of efficient recombinant proteins, in particular recombinant antibody molecules, preferably antibody fragments such as miniantibodies. . An E. coli expression vector suitable for expressing a foreign protein under high cell density fermentation conditions comprises at least (i) an upstream terminator sequence and a downstream terminator sequence, (Ii) lac promoter / operator system, (Iii) T7glO Shine Dalgarno sequence, (Iv) A pelB or ompA signal sequence , and (v) a foreign gene sequence. [Selection] Figure 2 |