http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007143435-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f97f0fa258a008bf056ad04b7f380dda |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 2005-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e3da148f0528165dae70c9099274ed9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1e67a5ca51387907b74d01b3763253c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e03c4e3d3a58b944ae954c4e67d9da3b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54fa021da98c2b212e4f85e7b9866b66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f9b3c332d10d05e27b67a238def90c5 |
publicationDate | 2007-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2007143435-A |
titleOfInvention | Cell-free protein synthesis method |
abstract | A novel cell-free protein synthesis method capable of achieving high throughput, and a method for producing a protein in a cell-free system using an insect cell extract or a mammalian cell extract Provide a kit to do In a translation reaction solution obtained by mixing a transcription reaction step in vitro, a transcription reaction solution after the transcription reaction, a solution for translation reaction, and a chelating agent that forms a complex with magnesium ions. A cell-free protein synthesis method, wherein the chelating agent is mixed so that a final concentration of free magnesium ions in the translation reaction solution is 10 mM or less. A reagent kit comprising at least a chelating agent that forms a complex with magnesium ions for performing the cell-free protein synthesis. [Selection] Figure 1 |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013009623-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017131169-A |
priorityDate | 2005-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 221.