abstract |
The present invention provides a cutinase which is a mutant of cutinase which is an esterolytic enzyme and has excellent thermostability, a gene encoding the same, a method for decomposing plastics using the mutant cutinase, and the like. . A) Mutants of cutinase in which an additional peptide consisting of 11 or less amino acids is bound to the amino terminus of the parent cutinase, and / or b) At least one amino acid residue corresponding to the following amino acid residues in the amino acid sequence of the cutinase of Aspergillus oryzae strain RIB-40: L2, G4, L8, P27, G28, A51, G54, K90, or A177 A variant of a parent cutinase comprising a substitution or deletion of the above, wherein the variant has better thermal stability than the parent cutinase. [Selection] Figure 2 |