http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007089548-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7e9aee13868bfedebe2df1f13cda5490 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 |
| filingDate | 2005-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69bc41fdd55e5e88d7cc69b367df72a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0192e31fceff5588d2195c78ff7ae495 |
| publicationDate | 2007-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2007089548-A |
| titleOfInvention | Signal amplification method |
| abstract | The present invention provides a novel enzyme-labeled tyramide that can be effectively used in a signal amplification method (TSA method) using tyramide. A signal amplification method using the enzyme-labeled tyramide is also provided. Tyramide is labeled with thermostable alkaline phosphatase or thermostable peroxidase to form thermostable enzyme-labeled tyramide. One of the signal amplification methods of the present invention can be carried out using the thermostable enzyme-labeled tyramide by the following steps; (1) a primary labeled molecule (for example, biotin) that specifically binds a target molecule to the molecule. (2) a step of binding a peroxidase-labeled primary conjugate to a primary label molecule (for example, a biotin-labeled molecule) bound to the target molecule, and (3) heat resistance in the presence of hydrogen peroxide. A step of reacting a sexenzyme-labeled tyramide or a functional equivalent thereof, and (4) a step of reacting the thermostable enzyme with a chromogenic substrate. [Selection figure] None |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CZ-300860-B6 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2011514522-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113030348-A |
| priorityDate | 2005-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 72.