| abstract |
PROBLEM TO BE SOLVED: To provide a soil diagnosis method for making soil before cultivating a crop, preventing soil diseases caused by parasitic nematodes, and allowing the farmer to produce the crop with peace of mind. SOLUTION: A soil disease is diagnosed by confirming the presence or absence of a parasitic nematode in the soil by separating the nematode from the soil, amplifying the DNA of the nematode by a PCR method, and performing electrophoresis. . Alternatively, by separating the nematode from the soil, amplifying the DNA of the nematode by a quantitative PCR method, and performing electrophoresis, the ratio of the parasitic nematode to the total number of nematodes in the soil is confirmed, thereby confirming the soil disease. Diagnose. [Selection figure] None |