http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007063194-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a6638ac6b1020cd2c62d09d748bc1ab9 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K9-127 |
filingDate | 2005-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ab3f4d8d04d07db8deab9cff5d09347 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df9f0a264d8182bdb30ff068ae08e4e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c965f63f4466fdf587beb9f6043ee84 |
publicationDate | 2007-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2007063194-A |
titleOfInvention | Substance introduction carrier, substance introduction method and substance introduction kit |
abstract | PROBLEM TO BE SOLVED: To provide a liposome-based substance introduction carrier, a substance introduction method, and a substance introduction kit capable of introducing a substance into cells with high efficiency. SOLUTION: Heat-denatured mRNA and cationic lipid DOTAP are dissolved in bicarbonate-buffered DMEM medium, incubated at room temperature for 15 minutes, added with CaCl 2 and incubated at 37 ° C. for 30 minutes, An mRNA-liposome-carbonate apatite particle complex that is an mRNA introduction carrier is prepared. By incubating this mRNA-liposome-carbonate apatite particle complex with target cells, mRNA can be introduced and expressed with high efficiency. [Selection] Figure 1 |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018199121-A1 |
priorityDate | 2005-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.