http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007061057-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b7a4fe316cda97fb7c41b3f2fcc41eb1 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-06 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 |
filingDate | 2005-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ccfe454314f69c05de3faba91e56229 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6980a2beb57303659ea3a25c6c337b26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a776465cf0d9a72d0bb30c6fd0c9d448 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf684194680bf18bdc48358650ee4c6b |
publicationDate | 2007-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2007061057-A |
titleOfInvention | Polypeptide production method and kit |
abstract | Disclosed is a method for producing a polypeptide that can suppress degradation of a target protein and that does not affect the cleavage efficiency depending on the amino acid sequence immediately after the cleavage site, and that uses a method for decomposing a novel tag and the target protein. Provided is a method capable of producing a polypeptide having the same amino acid and three-dimensional structure as a protein. A fusion polypeptide comprising a tag amino acid sequence, a GLD protease cleavage amino acid sequence and a target amino acid sequence is prepared, GLD protease is allowed to act on the fusion polypeptide, and the target amino acid is cleaved by the GLD protease cleavage amino acid sequence. A method for producing a polypeptide, comprising recovering a polypeptide containing a sequence. A method for producing a polypeptide, comprising culturing a host transformed with a DNA encoding Congelin II and an expression vector containing a DNA encoding a target amino acid sequence downstream of the DNA, and recovering the polypeptide having the target amino acid sequence. [Selection figure] None |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8481310-B2 |
priorityDate | 2005-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 194.