| abstract |
A high expression system of a target gene in a microorganism belonging to the genus Streptomyces is provided. The DNA construct of the present invention comprises an H-nitrile hydratase gene promoter or a variant thereof, a gene encoding a H-nitrile hydratase transcription regulatory protein or a variant thereof, a gene encoding a transcriptional activation protein, or a combination thereof. It includes a mutant, a multiple cloning site, and a terminator or a mutant thereof. [Selection figure] None |