http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007028923-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e2ecc443bf0fc450ba7ff3a5bd8ab61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_faf522b8b83eded745c73018576219ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bd89c0e07c12d4c43d22eb44b082d754 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06F19-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06F19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06F19-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2005-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd1900739452d9362d37d4edfc7d0873 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d92a37059df8edf6346a7ff8adc39b4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2245406cab2ca0b95951569c41352ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c3f74e2b462aec845a38dbda6e04ba83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3afe5ec54b72c19c0977a52fd9dbed72 |
publicationDate | 2007-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2007028923-A |
titleOfInvention | Method for producing single-stranded gene tag group including transcription initiation site |
abstract | An object of the present invention is to provide a method for producing a single-stranded gene tag group reflecting the type and quantitative ratio of the base sequence group of mRNA 5 ′ end extracted from a eukaryotic cell. Also provided is a method for measuring the expression level of a gene in a eukaryotic cell, comprising the step of hybridizing the single-stranded gene tag group to a solid phase on which DNA or RNA containing a transcription initiation site is immobilized. In addition, the gene expression information obtained is integrated to provide a method for creating a gene expression profile. SOLUTION: By combining the method disclosed in the 5'SAGE method with a technique for making a double-stranded DNA into a single strand, one that reflects the type and quantity ratio of the base sequence of the 5 'end of mRNA. As a result of producing a strand gene tag group and performing hybridization to a DNA chip using this as a sample, it was found that the single strand gene tag group was effective in confirming the expression of a gene targeting the expression start site. By using this single-stranded gene tag group, it becomes possible to perform comprehensive expression analysis of genes targeting various transcription initiation sites. [Selection figure] None |
priorityDate | 2005-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1069.