| abstract |
Purine or pyrimidine base and structure: -O-Z (In the formula, Z represents —C (R ′) 2 —O—R ″, —C (R ′) 2 —N (R ″) 2 , —C (R ′) 2 —N (H) R ′. ', -C (R') 2- SR "and -C (R ') 2- F, Where each R ″ is a removable protecting group or part thereof; Each R ′ is independently through a hydrogen atom, alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a linking group. Or a detectable label attached; or (R ′) 2 represents an alkylidene group of the formula = —C (R ′ ″) 2 , where each R ′ ″ may be the same or different, and hydrogen and Selected from the group comprising halogen atoms and alkyl groups; and wherein the molecule reacts and F is replaced when each R ″ is replaced with H or Z is —C (R ′) 2 —F. May produce an intermediate that is exchanged with OH, SH or NH 2 , preferably OH, which dissociates under aqueous conditions to yield a molecule with free 3′OH; However, when Z is —C (R ′) 2 —S—R ″, both R ′ groups are not H) A modified nucleotide or nucleoside molecule comprising a ribose or deoxyribose sugar moiety having a removable 3'-OH blocking group covalently linked thereto such that the 3 'carbon atom is attached to the present invention Will provide. |