http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006500959-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2003-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2006-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2006500959-A |
| titleOfInvention | Polynucleotide synthesis and labeling by dynamic sampling binding |
| abstract | A method for constructing and / or labeling a polynucleotide, and a kit therefor, comprising the binding of two or more oligonucleotides in the presence of a backbone oligonucleotide complementary or partially complementary to the oligonucleotide to be bound. A full-length oligonucleotide template is not required. The coupling can be performed under conditions that do not form a stable duplex between the backbone oligonucleotide and at least one oligonucleotide to be coupled. Under these unstable binding conditions, cleaved or unbound contaminants in these two oligonucleotide preparations do not significantly inhibit the formation of the desired oligonucleotide product. By the method of the present invention, the oligonucleotide in the binding reaction can be used without purification. The method of the present invention also allows for end-specific addition of oligonucleotides to one or both ends of the target oligonucleotide. |
| priorityDate | 2002-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 236.