| abstract |
The present invention relates to a novel set of four fluorescently labeled dye terminators. Two of these are single die label terminators and the other two die terminators are based on Foster resonance energy transfer (FRET). The FRET dye terminator is generated from 4 ', 5'-bis-aminomethyl fluorescein. Of the two amino groups of the donor dye 4 ', 5'-bis-aminomethyl fluorescein, one amino group is used to bind to the acceptor dye, and another one of the amino groups is dideoxynucleoside-5'. -Used to bind to triphosphate. Disclosed is its preparation, energy transfer efficiency and use as a label, particularly as a label in a DNA sequencing reaction. |