| abstract |
A creatinine biosensor that can be easily manufactured without impairing enzyme activity. In a sensor having at least a pair of working electrode and counter electrode on a substrate, a reagent solution in which creatininase, creatinase, sarcosine oxidase and mediator are dissolved in a pH 7 to 8.5 buffer solution is placed on the electrode. And / or a creatinine biosensor immobilized by drying on a substrate around the electrode. In this creatinine biosensor, a buffer is placed on the electrode in a solid state together with each enzyme of creatininase, creatinase, and sarcosine oxidase, so a buffer solution for stabilizing the enzyme during measurement is prepared. Therefore, the measurement sample can be used as it is, and an excellent effect is obtained that creatinine can be measured without requiring a complicated operation. [Selection figure] None |