http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006345703-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_32305ca3a8c0b7e8cff4bd2dcd2baf65 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-108 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2003-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da2790e7eb4fff94e383e1d3bd0b621a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a99b0127fe991c2f9c8d1f96054ad0fe |
| publicationDate | 2006-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2006345703-A |
| titleOfInvention | High expression general-purpose mammalian host cells |
| abstract | PROBLEM TO BE SOLVED: To provide a method for obtaining a gene amplification host cell having versatility, simply and rapidly, and a mammalian cell obtained by such a method. The present invention provides a mammalian cell having a plurality of sites into which a vector containing any foreign gene can be inserted by specific site-specific recombination. Specifically, according to the present invention, an expression vector containing a first site-specific recombination sequence and a selection marker is introduced into a mammalian cell, and the gene and nucleic acid sequence contained in the vector correspond to the selection marker. Gene amplification is performed using a gene amplification inducer, and then a vector containing a second site-specific recombination sequence and a foreign gene is introduced, and the first site-specific recombination sequence and the second site-specific combination are introduced. Mammalian cells produced by site-specific recombination with a replacement sequence by the action of a site-specific recombination inducer are provided. [Selection figure] None |
| priorityDate | 2003-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 836.