http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006296350-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9019c0ce5cb7b82c85e121f6cac86dce |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2005-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_247bee7b64a90ad7869bbf3dc4725671 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1487a2491961286f88e7ff45eeefb5bb |
| publicationDate | 2006-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2006296350-A |
| titleOfInvention | Method for producing nucleic acid construct in which a plurality of nucleic acids are linked and cloning of nucleic acid using the method |
| abstract | 【Task】 A nucleic acid construct is produced by linking a plurality of nucleic acid molecules without using a restriction enzyme. [Solution] The present invention includes the following: (a) a step of amplifying a first nucleic acid molecule to obtain a first double-stranded amplification product; (b) a second nucleic acid molecule being amplified to obtain a second double-stranded amplification product Wherein the 3 ′ end portion of the first double-stranded amplification product and the 5 ′ end portion of the second double-stranded amplification product are hybridized only with a specific sequence. (C) mixing the first and second double-stranded amplification products obtained by steps (a) and (b), and A step of denatured into a strand and hybridizing under a condition in which only the specific sequence hybridizes; and (d) a nucleic acid amplification reaction using the mixture of (c) as a template to obtain a linked nucleic acid amplification product. A method comprising the steps is provided. [Selection] Figure 2 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008115101-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008115092-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009165371-A |
| priorityDate | 2005-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 494.