http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006262797-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a7b097bfd3f350ed27ffa53e1f114d1a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2005-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91297014fa2a31082e3a2dd36ef1f943 |
| publicationDate | 2006-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2006262797-A |
| titleOfInvention | Method for detecting gene expression using a capillary array |
| abstract | The present invention provides a method for analyzing gene expression that can be carried out with high sensitivity using a small amount of nucleic acid probe and that can be carried out safely and simply because no radioisotope is used. A step of immobilizing a probe complementary to a target gene and containing a first labeling substance on a capillary array; an extension reaction solution for incorporating a second labeling substance into a nucleus isolated from a cell; A step of resuming the extension reaction to produce a labeled nucleic acid; a step of extracting the labeled nucleic acid; introducing the labeled nucleic acid into the capillary array and hybridizing the probe and the labeled nucleic acid Degrading the non-hybridized probe with a single-strand-specific nucleolytic enzyme; removing the degradation product from the capillary array; and remaining first and / or second labeling substance And a step of detecting. [Selection] Figure 1 |
| priorityDate | 2005-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 101.