http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006262779-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_24aca9ded2638ea793d05360dde7a4a0 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2005-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_550a5277eedca8b1a2d1c6b1e20a66c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_edebf9e911c2c197705c72b2a0409ac7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7bdf96773a70c853c9e52eddbc9bd196 |
| publicationDate | 2006-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2006262779-A |
| titleOfInvention | Method for obtaining a crystal comprising a complex of a glucocorticoid receptor ligand binding domain and a ligand |
| abstract | 【Task】 It is possible to provide a method for obtaining a crystal comprising a complex (complex) of a glucocorticoid receptor ligand binding domain (domain) and a ligand (ligand) having a non-steroid skeleton. [Solution] The microorganism expressing the fusion protein of the purified domain and the domain is cultured in the absence of a ligand, and the fusion protein is recovered from the collected microbial cell disruption based on the binding properties of the purified domain and the carrier for purification. The protease is cleaved with a protease by cleaving the protease-cleavable linker (linker) present in the purified domain, and the microorganism is separated in the step before the linker is cleaved with the protease in the crushing / collecting / separating step. Alternatively, “a method for obtaining a crystal containing a complex”, in which a crystal obtained by subjecting a complex obtained by “a method for obtaining a complex” to contact a cell or a fusion protein and a ligand is subjected to crystallization treatment. [Selection figure] None |
| priorityDate | 2005-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 86.