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filingDate 2003-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2005-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2005516213-A
titleOfInvention Bacterial spore analysis method and apparatus
abstract A sample of unknown bacterial spores is added to the test strip. A sample of unknown bacterial spores is drawn to the first sample area on the test strip by capillary action. When the unknown bacterial sample matches the species-specific antibody, the species-specific antibody is sampled, otherwise the sample is left unbound. DPA is released from the bacterial spores of the bound sample. Terbium ions are combined with DPA to form a Tb-DPA complex. The bound terbium ion and DPA are excited to produce luminescence characteristic of the bound terbium ion and DPA, and bacterial spores are detected. Live / death assays are performed by release of DPA for live spores and release of DPA for all spores. The detected concentration is compared to determine the percentage of viable spores. Removing the fluorescent background from the organic chromophore by making a lifetime-gated measurement of the bacterial spore labels the bacterial spore contents with long-lived lumophore and luminescence after waiting time Detecting. Unattended monitoring of bacterial spores in the air involves collecting the bacterial spores carried in the air and repeating the steps of Tb-DPA detection described above. The present invention is also an apparatus for performing the various methods disclosed above.
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