http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2005514065-A
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2003-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2005-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2005514065-A |
titleOfInvention | In vivo nucleic acid level determination method |
abstract | PROBLEM TO BE SOLVED: To collect a biological sample in a collection tube containing a compound that specifically suppresses RNA degradation and / or gene induction; generates a precipitate containing nucleic acid; and separates the precipitate from a supernatant; To dissolve the precipitate and form a suspension; to isolate nucleic acid from the suspension using an automated instrument; to isolate nucleic acid from the suspension; to mix for RT-PCR using an automated instrument Disperse / distribute reagents; disperse / distribute isolated nucleic acids in dispersed mixed reagents using automated equipment; use in-device nucleic acid / RT-PCR mixed reagents to in vivo levels of transcripts The present invention relates to a determination method of RNA in vivo from a biological sample. The invention also relates to the quantification of DNA from biological samples. The invention further clarifies a kit for the isolation of quantifiable nucleic acids from biological samples. The application of the method described in the present invention is also disclosed. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009081854-A1 |
priorityDate | 2002-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 464.