http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2005514003-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-113 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2002-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2005-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2005514003-A |
| titleOfInvention | Low temperature cycle extension of DNA with high priming specificity |
| abstract | The present invention relates to a method of extending a primer or primer pair in low temperature cycle DNA amplification for cycle sequencing and PCR. In particular, the method uses a combination of moderately thermostable DNA polymerases in the presence of low concentrations of glycerol or ethylene glycol or mixtures thereof as agents that lower the melting point of DNA (ie, the temperature at which double-stranded DNA is denatured). Contemplate. Also contemplated by the present invention is a pre-distributed, high fidelity and high processivity DNA polymerase reaction mixture that is stable for several weeks at room temperature in a kit prepared for immediate use. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010246529-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014239691-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016036304-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2010246528-A |
| priorityDate | 2001-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 133.