| abstract |
Methods, systems, compositions and kits for improved detection of polynucleotides. In one aspect, a liquid chromatography separation device (eg, reverse phase column or ion exchange column) is used to contact the eluted polynucleotide with an intercalating dye and to detect the dye bound to the eluted polynucleotide ( Methods are provided for separating polynucleotides (eg, DNA or RNA), eg, by fluorescence detection. The present invention preferably uses a post-column reactor such as a mixing tee device downstream of the separation column. Increased sensitivity of mutation detection by denaturing high performance liquid chromatography (DHPLC). |