| abstract |
The present invention provides a novel recombinant fusion protein for detecting binding of a molecule of interest comprising a detection domain, a first and optionally a second localization domain, and a binding domain. The present invention also provides recombinant nucleic acid molecules and recombinant expression vectors encoding these novel fusion proteins, genetically engineered host cells containing these expression vectors, and these fusion proteins, nucleic acid molecules, expression vectors, and hosts Kits for the use of cells are also provided. Furthermore, the present invention provides a method for identifying a compound that alters the binding of a molecule of interest in a cell. |