| abstract |
PROBLEM TO BE SOLVED: To screen for an agent that inhibits intracellular phosphodiesterase 10A activity, comprising administering the agent to striatal medium spiny neurons, and Adenylate cyclase is submaximally activated, the agent is administered to striatal medium spiny neurons, and guanylate cyclase is submaximally activated to measure cAMP and cGMP production in cells, and cAMP EC 200 and calculating cGMP EC 200 , wherein the agent is identified as a PDE10A inhibitor if the ratio of cAMP EC 200 / cGMP EC 200 is comparable to the ratio produced by administration of papaverine under the same assay conditions, Features method. Also featured are rat PDE10A polynucleotide and polypeptide sequences. [Selection figure] None |