| abstract |
Disclosed is a method for obtaining novel polynucleotides and encoded polypeptides by using a non-stochastic method of directed evolution (DirectEvolution ™ ). Non-probabilistic polynucleotide site saturation mutagenesis (Gene Site tion Mutagenesis ™ ) and non-stochastic polynucleotide reassembly (ssembly ™ ) are included. Genetic vaccines, enzymes, and other desired molecules can be evolved in the direction of the desired properties. For example, a vaccine vector with increased efficacy as a genetic vaccine is obtained. [Selection figure] None |