| abstract |
PROBLEM TO BE SOLVED: To provide an enzyme having a lytic activity against the cell wall of Saccharomyces cerevisiae, a method for producing the enzyme, a microorganism producing the enzyme, a method for using the enzyme, and the like. In order to obtain yeast cell wall lytic enzyme, microorganisms having high ability to produce Saccharomyces cerevisiae cell wall lytic enzyme were screened from soils in various parts of Japan. The production enzymes of the selected strains were roughly purified, and the production enzymes β-glucan and pNPG (p-nitrophenyl-β-glucoside) were evaluated for degradability. In addition, the effects of temperature and pH on the production enzyme were examined. Moreover, the yeast cell wall lytic enzyme-producing bacterium KH-3 strain was identified, and it was confirmed that the KH-3 strain belongs to the cellulocymicrobium cerance. |