| abstract |
An enzyme having cephalosporin C acylase activity, a recombinant DNA method for preparing the enzyme, a nucleotide sequence encoding the enzyme, an expression vector containing the nucleotide sequence, and a transformation with the expression vector To provide a method for preparing 7-amino-cephalosporanic acid with cells and said enzyme. An enzyme having cephalosporin C acylase activity obtained by native sequence site-directed mutagenesis, random mutagenesis or “site saturation” mutagenesis optimized for expression in E. coli. [Selection] Figure 1 |