| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ee1c19da359446fb5c4f0458a57b783d |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2004-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8401dfc55e43cffb49981913685442c0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_711d90023e925a32b51c23969b0c3345
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b54cc780718c172351b956f3c88f643f |
| publicationDate |
2005-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2005198612-A |
| titleOfInvention |
Yeast transformation method |
| abstract |
The present invention provides a yeast transformation method that does not require adjustment of competent cells and can easily and easily treat a large number of strains. [MEANS FOR SOLVING PROBLEMS] More than 1 × 10 7 yeast cells cultured on a solid medium are added to a treatment solution comprising a transformation DNA, carrier DNA, polyethylene glycol and a lithium salt-containing buffer per μg of transformation DNA. Incubating for 30 minutes or more, and the treatment solution contains 0.5 to 10 μg of DNA for transformation per 100 μl thereof, and carrier DNA contains 20 times or more mass of the DNA for transformation. A method for producing a transformant by transforming with a DNA for transformation. [Selection] Figure 1 |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012060389-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011021629-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9284561-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012128260-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013111754-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015076393-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010038802-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014030644-A1 |
| priorityDate |
2004-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |