| abstract |
The present invention provides a production system for a substance having low heat stability using a psychrophilic microorganism as a host. An expression vector containing a DNA encoding a target protein in a state capable of being expressed in a chilled microorganism is introduced into the chilled microorganism, the chilled microorganism is cultured, and the protein is collected from the culture. To produce protein. As the expression vector, a vector that includes a DNA encoding a target protein, a promoter that functions in a psychrophilic microorganism, and a replicon that functions in the psychrophilic microorganism operably linked is used. [Selection figure] None |