| abstract |
【Task】 The binary probe and clamp composition implements a method for target hybridization detection. [Solution] Where the probe is a substrate for exonuclease cleavage, the composition provides quantification and detection of PCR products by real-time and endpoint measurements. Where the probe is an amplification primer, the composition provides an improved method for labeling and detecting PCR products. Probes and clamps can be labeled with fluorescent dyes, quenchers, hybridization stabilizing moieties, chemiluminescent dyes, and affinity ligands. The clamp can be a nucleic acid analog such as 2-aminoethylglycine PNA. |